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Abstracts
Water extract of Turmeric acts like a plant growth hormone
Dr. Leela Srinivas, Director, BGS-ABCRI
(1). Healthy Turmeric plantlets grown in tissue culture derived from healthy rhizome callus or robust rhizomes grown in soil were treated with 10 ml of 10% clear turmeric water extract with 95 ml of water per day at regular intervals. Plant height, number of shoots and leaves monitored at regular intervals.
Over a period of a month, untreated turmeric plant had 4 to 6 leaves and 2 shoots, whereas, the turmeric water extract treated plant had 6 to 8 leaves and 3 shoots. The height of the plants in both groups was around 15 cm and at the beginning and at the end of 4 months, the untreated was around 35 cms height where as turmeric extract treated plant was around 50cms height.
From the above observation, It appears that, water extract of Turmeric may act as a plant growth hormone. The yield of rhizomes in the treated group were more in numbers as well as robust plants. This possibly indicates that, treated Turmeric plant will give better yield and thus, may help the farmers to book higher profits.
(2). The above observation was extended to other plants namely Fenugreek and Ragi. Interestingly both the plants showed luxurious growth when treated with Turmeric water extract and resisted fungal infection. It appears that, probably all the plants will respond positively to treatment with Turmeric water extract, thus, possibly helping the farmers to increase yield, grow healthy plants and derive more profit.
CANCER CURED !!!
Dr. Leela Srinivas
The following are two heartening stories to be shared with the browsers of our websites www.bgsabcri.org and www.turmericglobal.net
A middle aged man residing at Chamarajanagara – a town near Mysore was suffering from throat cancer and was diagnosed as being in the 4th stage of the disease. He was advised by his friend, a regularly visitor of our website, and hence knew what to do. He recommended that the patient to take ½ teaspoon of Turmeric powder in water in the morning on an empty stomach three times a week. Low and behold ! after three months of regular intake of Turmeric, his cancer has disappeared. He now walks the streets of Chamarajanagara as a happy person. However, this is an anecdotal incident, and the above person is the 11th cancer patient cured by turmeric treatment.
"TURMERIC CURES FUNGAL INFECTION"
Dr. Leela Srinivas
A gentleman of 50 years old, residing at Davanagere - a big city in Karnataka, India had a problem of constant, profuse sweating in his hands. He being an employee of a court had to handle old dusty documents. This handling caused the infection of Candida albicans as diagnosed by physician and he was using standard antifungal ointment without much benefit. He was advised by one of our Ph.D. student as to the antifungal property of water extract of Turmeric. Accordingly he started immersing both his hands in clean, clear warm water extract of Turmeric powder thrice a day for about a week regularly. At the end of the period, he found complete disappearance of fungal infection. At this time it is also appropriate to recollect that, an farmer being cured of fungal infection of the eye by fomenting of pure and clear turmeric powder tied in a clean cloth to the infected eye which proved better than the antibiotic he was using and was cured.
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BGS-Haridrin : A glycoprotein from boiling water extract of Turmeric (Curcuma longa L)
Dinesha R. and Leela Srinivas
Adichunchanagiri Biotechnology and Cancer Research Institute, Balagangadharanatha Nagara-571448, Mandya District, Karnataka, INDIA
Corresponding Author
Dr. Leela Srinivas, Ph.D. Adichunchanagiri Biotechnology and Cancer Research Institute, Balagangadharanatha Nagara-571448, Mandya District, Karnataka, INDIA Email: directorabcri@yahoo.com
BGS-Haridrin - a glycoprotein isolated from boiling water extract of Turmeric (Curcuma longa L). The extract of turmeric gave three peaks (P-I, P-II and P-III) in Sephadex G-25 chromatography. P-I showed antioxidant activity and hence subjected to DEAE A-25 chromatography showed five peaks. Peak IV showed antioxidant activity, this was named as BGS-Haridrin. SDS-PAGE and MALDI/MS analysis showed its molecular weight as ~28 kDa. BGS-Haridrin at 0.9 nM concentration shows 82%, 80%, inhibition in hydroxyl radical scavenging and lipid peroxidation inhibition assay respectively. BGS-Haridrin provides protection against H2O2, t-BOOH induced DNA damage and also against Beedi, Cigarette and Cow dung smoke condensate carcinogens and reactive oxygen species induced DNA damage. Our protein showed antibacterial activity against strains like Staphylococcus aureus, Proteus vulgaris, Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium, Vibrio cholerae, Streptococcus sps and Pseudomonas sps. MIC values of BGS-Haridrin (3.25µM) was eight time lesser than Streptomycin (25.8 µM). BGS-Haridrin was also found to be effective against the fungi Candida albicans. MIC of BGS-Haridrin (1.5µM) was 16 times lesser than Amphotericine (23.8µM).
Thus, the present study demonstrates the in vitro antioxidant, DNA damage protectant activity and antibacterial of BGS-Haridrin.
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ANTIMICROBIAL AND ANTIPLASMODIAL ACTIVITY OF ARISHININ: AN ANTIOXIDANT PEPTIDE ISOLATED FROM CURCUMA LONGA LINN.
Harsha Ramakrishna and Leela Srinivas
Adichunchanagiri Biotechnology and Cancer Research Institute (ABCRI), Balagangadharanatha Nagara-571448, Mandya District,Karnataka, INDIA.
Corresponding Author
Dr. Leela Srinivas. Ph.D.
Director, Adichunchangiri Biotechnology and Cancer Research Institute, Balagangadharanatha Nagara - 571448, Nagamangala Taluk, Mandya, Karnataka, INDIA.
Tel: +918234-287850, Fax:+918234-287984 Email: directorabcri@gmail.com
Arishinin, an antioxidant peptide (Mol wt:~8.2kDa) isolated from boiling water extract of turmeric rhizome (Curcuma longa linn). It is an effective ROS quencher and at 0.73µM it displays 87 and 85% inhibition in TBARS and hydroxyl radical induced oxidative stress. Against DPPH and Nitric oxide free radicals, at 1.87µM, it showed 82 and 89% inhibition respectively and on a comparative scale to the standard antioxidants like Curcumin, a-Tocopherol and BHA, arishinin was required only at 10-20 times lesser dose. Antimicrobial activity by Disc diffusion and MIC value by Serial dilution was done against microbes viz E. coli, V. cholera, K. pneumonia, S. aureus, S. typhimurium, Pseudomonas, P .vulgaris, C. albicans, A. flavus, T .mentagranophytes and Rhizobium. Streptomycin and Amphotericin-B was used as standard antibacterial and antifungal agents. Average MIC values of Arishinin against bacterial and fungal strains were 2.5 and 6µM and it was 10 and 5 times lesser than the standard antibiotics like Streptomycin and Amphotericin-B. Against the malarial vector, Plasmodium falciparum, Arishinin was effective on both Chloroquine-resistant RKL-9 and Chloroquine-sensitive MRC-2 strains with an EC50 of 24 and 27nM where as Curcumin showed 50% inhibition at 5µM concentration. Arishinin was 208 and 185 times better than Curcumin against CQ-res and CQ-sen strains of P.falciparum respectively.
Arishinin, a peptide isolated from Curcuma longa is a novel Antioxidant, Antimicrobial and Antiplasmodial non toxic peptide with a possible preventive/curative health benefit.
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Isolation and characterization of hemostatically active components from cold water extract of Curcuma longa Linn. rhizome
Shivalingau B.R. and Leela Srinivas
Adichunchanagiri Biotechnology and Cancer Research Institute
Corresponding Author
Dr. Leela Srinivas. Ph.D. Director, Adichunchangiri Biotechnology and Cancer Research Institute, Balagangadharanatha Nagara - 571448, Nagamangala Taluk, Mandya, Karnataka, INDIA. Tel: +918234-287850, Fax: +918234-287984 Email: directorabcri@gmail.com
Traditionally in India turmeric is applied on cuts to stop the bleeding. The crude cold water extract of Curcuma longa Linn. exhibits proteolytic activity, as estimated by the caseinolytic assay. Casein hydrolyzing activity of a protease was inhibited by PMSF indicating that the protease possibly belongs to serine protease family. The crude extract also exhibits pro-coagulant activity by reducing plasma clotting time from 235 to 66s and promotes blood coagulation. It also hydrolyses human fibrinogen Aα and then the Bβ subunits, whereas γ subunit is resistant. The extract also shows plasmin like activity as it hydrolyses fibrin clot subunit which is cross linked α-polymer and the partial hydrolysis of γ - γ dimer. The α and β chain of fibrin appears to be resistant. The hydrolysis of human fibrinogen and fibrin clot by crude rhizome extract implies significant hemostatic property in relevance to wound healing. This study gives the scientific credence to C. longa with its propensity to stop bleeding and wound healing. However it is noteworthy that the boiling water extract of C. longa containing the antioxidant proteins-Arishinin of 8kDa, Turmerin of 14 kDa (UNIPROT P-85278) and BGS-Haridrin of 28kDa does not show any protease activity and on the other hand, they are resistant to pepsin and trypsin.
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